Differential subcellular localization of ectopic Bβ2 and Bβ1 by confocal image analysis. (A) Cells were immunostained with antiserum against β2 (red) and counter-stained with mitotracker (green) and visualized by confocal microscopy. The merged graph showed that β2 was colocalized as yellow colors at mitochondria in clone Bβ2 #5 and #8. (B) Cells were immunostained with antiserum against β1 (red) and mitotracker as before. The merged graph showed that ectopic β1 can be distributed in cytoplasm in clone Bβ1#1 as compared with SK-N-SH cells. Bar, 20 μm.