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Table 1 Expression and/or phosphorylation activation of proteins in PDL cells subjected to strain denoted in percentage

From: Mechano-transduction in periodontal ligament cells identifies activated states of MAP-kinases p42/44 and p38-stress kinase as a mechanism for MMP-13 expression

Molecule control 0 h 0.25 h 0.5 h 1 h 3 h 6 h
integrin β3 (Int β3) / ± 0 ± 0 ± 0 ± 0 +32.2% * p < 0.01
focal adhesion kinase (FAK) / ± 0 ± 0 ± 0 ± 0 + 28.7% * p < 0.01
phosphorylated focal adhesion kinase (pFAK) / + 12.8% * p < 0.01 ± 0 ± 0 ± 0 ± 0
p42/44 / + 5.8% * + 12.5% * p < 0.01 ± 0 - 9.0% * p < 0.01 + 0.2% *
phosphorylated p42/44 / + 2.3% * - 7.3% * -10.0% * p < 0.01 + 0.5% * + 10.2% * p < 0.01
phosphorylated p42/44 + inhibitor / / / / / maximum inhibition - 16.0% p < 0.01
p38 / - 5.3% * p < 0.01 - 13.8% * p < 0.01 - 9.6% * p < 0.01 - 3.1% * + 26.2% * p < 0.01
phosphorylated p38 / + 5.7% p < 0.01 - 1.7% + 3.1% + 0.9% + 11.4% p < 0.01
phosphorylated p38 + inhibitor / / / / / maximum inhibition - 62%
matrix metalloproteinase 13 (MMP13) / + 8.4% * p < 0.01 + 1.4% * +1.53% * + 0.7% * + 16.0% * p < 0.01
matrix metalloproteinase 13 (MMP13) + pp38 inhibitor - 11.0% p < 0.01 - 17.0% p < 0.01 - 18.8% p < 0.01 - 13.5% p < 0.01 - 12.0% p < 0.01 - 25.7% p < 0.01
matrix metalloproteinase 13 (MMP13) + pp42/44 inhibitor - 22.6% p < 0.01 - 28.6% p < 0.01 - 24.3% p < 0.01 - 24.8% p < 0.01 - 24.3% p < 0.01 -34.1% p < 0.01
  1. Densitometric measurements of western blot analysis were calculated in % of relative gray pixel values and normalised to control* or to the respective time point in the stretch kinetic. Date represent the mean of three independent experiments (n = 3) and means were subjected to the Students T-test. All compared mean values with p < 0.01 were considered as statistical significant.