Figure 2

Sequence alignment of AIF proteins and analysis of AIF expression. A. CLUSTAL-W was used to generate AIF sequence alignment, including Homo sapiens, Dictyostelium discoideum, Caenorhabditis elegans and Tetrahymena thermophila. Boxes indicate the NAD/FAD binding domain and oxidoreductase domain. MLS in N-terminal portion of T.thermohila indicates mitochondrial localization sequence. Asterisks indicate identical amino acids. Colons and semicolons indicate amino acid similarity. Arrowheads indicate a potential DNA binding site of human AIF. B. RT-PCR analysis of AIF transcript during conjugation. Histone h3 (HHT3) was used as a control. SSU rRNA was used as a loading control.