Figure 5

Progression of PND by disruption of AIF. A. Nuclear events during conjugation were divided into 6 stages (stage A ~stage F). B. Time course analysis of progression of the nuclear events in wild-type and ΔAIF between 6 h and 28 h after initiation of conjugation. The percentages of the nuclear stages were counted, and were expressed as a percentage of the total number of tested cells (300-400 cells). Columns and attached bars correspond to the means of four identical measurements and standard deviations. Fragmental DNA was isolated from the strains every 2 h during conjugation. The black stars between 8-12 h in ΔAIF indicate delay of kb-sized DNA fragmentation. M denotes kbp-ladder size marker (left) and 100-bp ladder size marker (right). C. Changes in size of parental macronuclei between 6 h and 28 h after initiation of conjugation. Columns (points) and attached bars correspond to the means of four identical measurements (80-100 cells) and standard deviations.