Figure 3

Sus1 co-localises with P-bodies. (A) Sus1 partially localises at P-bodies in stationary phase. Wild-type (wt) cells co-transformed with a plasmid containing the cDNA of SUS1 (pGFP-SUS1cDNA) and a plasmid containing Dcp2 (pDcp2-RFP) were observed in stationary phase. Partial co-localisation indicates localisation of Sus1 in P-bodies. Pictures were obtained by fluorescence microscope. (B) Sus1 is present at P-bodies independently of Lsm1 or Pat1. Cells expressing SUS1cDNA and Dcp2 in the different mutants were observed in stationary phase. Pictures were taken with a camera mounted onto a fluorescence microscope. (C) SUS1 is not required for P-bodies or stress granules formation. Cells expressing Dcp2-RFP or Pab1-GFP were transformed in sus1Δ and observed in stationary phase or after heat shock respectively. Images were generated using a fluorescence microscope.