The bipartite NLS directs nuclear localization of Bmp2. BALB/3T3 and 10T1/2 cells were transfected with wtBmp2/GFP or NLSmtBmp2/GFP, stained with DAPI (red in these images), and analyzed by ImageStream® imaging flow cytometry. (a) Representative images of cells expressing no GFP (top panel), and of cells with low (non-nuclear localization) and high (nuclear localization) similarity scores. (b) Overlay histograms comparing similarity scores from wtBmp2/GFP and NLSmtBmp2/GFP in BALB/3T3 and 10T1/2 cells. The percentage of cells with nuclear GFP was defined by gating the events with high similarity scores as shown by the horizontal lines in the top right of each histogram. Percentages of cells with nuclear GFP are given (upper right).