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Table 1 Cell speed variability caused by imprecise centroid selection

From: Significantly improved precision of cell migration analysis in time-lapse video microscopy through use of a fully automated tracking system

Cell type σ in μm original MD AMD in μm/min % AMD (smoothed) %
sc 3 0.181 0.423 234 0.125 69
sc 4.5   0.578 320 0.151 84
sc 6   0.747 414 0.181 100
sc 7.5   0.921 510 0.218 121
mc 3 0.622 0.723 116 0.465 75
mc 4.5   0.829 133 0.473 76
mc 6   0.956 154 0.484 78
mc 7.5   1.099 177 0.499 80
fc 3 1.787 1.821 102 1.589 89
fc 4.5   1.860 104 1.591 89
fc 6   1.923 108 1.597 89
fc 7.5   1.985 111 1.598 89
  1. Three cell types where tested (slow cell (sc), medium fast cell (mc), fast cell (fc)). The centroid positions where artificially varied within a standard deviation (σ, both axes) of 3 to 7.5 μm around the real centroid and the average mean displacement (AMD) computed for each set of varied tracks (200 tracks per setting). Variation of centroid positions resulted in overestimation of cell speeds, which was most pronounced for the slowest cell. Smoothing of "noisy" cell tracks by a centered moving average filter (window size 5) tended to underestimate MD values to varying degrees.