Figure 3
The Effects of Sal B on α-SMA and E-Cadherin expression in kidneys of model rats. (A) Immunofluorescence staining of α-SMA and E-cadherin in kidneys. Increased labeling of α-SMA in the tubule was observed in model rats, while it was noted to be decreased by Sal B treatment. The E-cadherin labeling intensity was decreased in model rats, whereas the intensity was maintained in the Sal B treatment group (B) Western blot analyses for α-SMA and E-cadherin expression in renal tissues. Significantly increased α-SMA expression and decreased E-cadherin expression were observed in rats of the model group. Sal B treatment attenuated HgCl2-induced upregulation of α-SMA expression. Moreover, it significantly inhibited the HgCl2-induced decrease of E-cadherin expression. (C) Graphic presentation of the relative expression of α-SMA and E-cadherin. The values are represented as the density of α-SMA or E-cadherin vs GAPDH (%). **P < 0.01 vs normal; *P < 0.05 vs normal; #P < 0.05 vs Model; # #P < 0.01 vs Model.