Table 1 Primers used to generate expression constructs
HA-GLT25D1-Myc (construct 1) |
|---|
Forward primer, GTGGGTACCATGTACCCATACGATGTTCCAGATTACGCCGCGGCGGCCCCACGCGC Reverse primer, TAGTCTAGAGAGAGTTCATCCCGGGCAGCAC |
GLT25D1-Myc (construct 2) |
Forward primer, GTGGGTACCATGGCGGCGGCCCCACGCGC Reverse primer as for HA-GLT25D1-Myc |
Δ signal sequence -GLT25D1-Myc (construct 3) |
Forward primer, GTGGGTACCATGGGCGCCGACGCCTACTTC Reverse primer as for HA-GLT25D1-Myc |
HA-GLT25D1 (construct 8) |
Forward primer as for HA-GLT25D1-Myc Reverse primer, TAGTCTAGATCAGAGTTCATCCCGGGCAGCAC |
HA-GLT25D1-ΔRDEL (construct 9) |
Forward primer as for HA-GLT25D1-Myc Reverse primer, TAGTCTAGATCAGGCAGCACTGTCCAGTGG |
GLT25D1-internal tag (construct 10) |
Oligo 1, GGAACAAAAACTCATCTCAGAAGAGGATCTGCA Oligo 2, GATCCTCTTCTGAGATGAGTTTTTGTTCCTGCA |