Figure 3
Late endocytic and lysosome-related organelles in developing intestinal cells of cup-5 mutant embryos. A) Confocal images of a cup-5(zu223) embryo that expresses YP170::GFP and stained with Nile Red. B) Confocal images of a cup-5(zu223) embryo that expresses YP170::GFP and immunostained to detect PGP-2. C) Epifluorescence images of a cup-5(zu223) embryo that expresses YP170::GFP and LMP-1::TagRFP(S158T). Small arrows point to organelles containing only YP170::GFP; large arrowheads point to organelles containing both YP170::GFP and LMP-1::TagRFP(S158T). D) Confocal images of a cup-5(zu223) embryo that expresses LMP-1::GFP and immunostained to detect PGP-2. Bottom panels are magnified images of the regions indicated in the top panels. Scale bars in whole embryo images represent 10 μm; scale bars in magnified images represent 2 μm.