Figure 4

cup-5 mutant effects on sizes and intensities of markers of late endocytic and lysosome-related organelles in developing intestinal cells. Images of wild type and cup-5(zu223) embryos, as well as quantification of sizes and intensities per unit area of compartments with YP170::GFP (A), LMP-1::GFP (B), PGP-2 (C), and UV (D) signals. Images for the same marker were taken using the same microscopy parameters. Scale bars represent 10 μm. Error bars represent the 95% confidence intervals for significance, and asterisks represent statistical significance at p < 0.05, compared to WT. In cup-5(zu223) embryos, the difference in areas of YP170::GFP organelles (A) with or without LMP-1::TagRFP(S158T) is also statistically significant.