The methylated N-terminal tail of RCC1 is required for stabilisation of its interaction with chromatin by Ran in live cells

Table 1 Stable binding of Ran with RCC1 regulates chromatin binding of RCC1 through the N-terminal tail in live cells.

Constructs	Mean FRAP half-time ± SD (s)	Mean mobile fraction ± SD	n
GFP + mCherry	0.08 ± 0.09	0.92 ± 0.06	19
GFP + mCherry-RanWT	0.08 ± 0.08	0.89 ± 0.04	14
GFP + mCherry-RanT24N	0.09 ± 0.09	0.91 ± 0.06	17
RCC1α-GFP + mCherry	2.93 ± 0.56	0.91 ± 0.05	21
RCC1α-GFP + mCherry-RanWT	2.85 ± 0.63	0.91 ± 0.04	26
RCC1α-GFP + mCherry-RanT24N	3.69 ± 0.58	0.87 ± 0.05	21
Δ27RCC1-GFP + mCherry	0.81 ± 0.21	0.94 ± 0.05	23
Δ27RCC1-GFP + mCherry-RanWT	0.87 ± 0.27	0.95 ± 0.04	23
Δ27RCC1-GFP + mCherry-RanT24N	0.87 ± 0.28	0.94 ± 0.04	26
RCC1αK4Q-GFP + mCherry	2.19 ± 0.44	0.90 ± 0.06	27
RCC1αK4Q-GFP + mCherry-RanWT	1.82 ± 0.54	0.90 ± 0.06	26
RCC1αK4Q-GFP + mCherry-RanT24N	2.19 ± 0.63	0.90 ± 0.07	30
RCC1αD182A-GFP + mCherry	1.51 ± 0.39	0.93 ± 0.04	20
RCC1αD182A-GFP + mCherry-RanWT	1.30 ± 0.54	0.94 ± 0.05	10
RCC1αD182A-GFP + mCherry-RanT24N	2.63 ± 0.62	0.87 ± 0.05	12

Fluorescence recovery after photobleaching (FRAP) data derived from live U2OS cells co-expressing GFP, RCC1α-GFP, Δ27RCC1-GFP, RCC1α K4Q-GFP, RCC1αD182A. The table summarises the mean +/- standard deviation (SD) of FRAP half-time and mobile fraction of each of the GFP fusion constructs in the nuclei of cells that also expressed mCherry, mCherry-RanWT or mCherry-RanT24N. The number of cells used in each case is given (n).

ISSN: 2661-8850