Figure 3

IGF2/IGFBP2 Promotes Human MSCs Osteogenic Differentiation. Adult hMSCs were treated with increasing concentrations of IGF2 and IGFBP2 for 4 days and alkaline phosphatase activity (ALP) was determined (A). Adult hMSCs were treated with or without IGF2 (2 nM) and IGFBP2 (0.5 nM) for 3 days, total RNA was collected and used for quantitative RT-PCR analysis of Runx2, ALP and Col1A1 expression (B-D). Adult hMSCs were cultured in an osteogenic differentiation medium, treated with/without IGF2 (2 nM), IGFBP2 (0.5 nM) or their combination and extracellular matrix mineralization was evaluated by alizarin red staining (E) and confirmed by quantification (F). Results are expressed as the mean ± SD of treated over control ratio. *: significant difference with untreated cells (P < 0.05).