Figure 2
From: Yeast karyopherin Kap95 is required for cell cycle progression at Start
Analysis of Start transcription in the kap95 mutant strain. (A) Northern analysis of the expression of the Start genes CLN2 and RNR1 in exponentially growing cells of the wild type (W303-1a) and tetO 7 :KAP95 (JCY635) strains incubated in the presence of 5 μg/mL doxycycline for 6 hours. SRC1 transcript is shown as a loading control. (B) Exponentially growing cells of the cdc15 and cdc15 tetO 7 :KAP95 (JCY1543) strains were incubated in the presence of 5 μg/mL doxycycline at 37° for 4 hours. After transfer to 25°, expression of CLN2 and RNR1 genes at the indicated times was analyzed by northern analysis (C) Exponentially growing cells of the tetO 7 :KAP95 (JCY635) strain transformed with a control plasmid (pRS313) or a plasmid expressing the CLN2 gene under the control of the S. pombe adh1 promoter were incubated in the presence of 5 μg/mL doxycycline for 6 hours. The graph shows the distribution of cells based on the bud size. (D) Exponentially growing cells of the cdc15 tetO 7 :KAP95 (JCY1543) strain transformed with a control (pRS313) or the adh1:CLN2 plasmid were incubated in the presence of 5 μg/mL doxycycline at 37° for 4 hours. After transfer to 25°, cell morphology was analyzed at the indicated times.