Figure 5

H ₂ O ₂ increased nuclear Oct-1 content, associated with reduced Cdx-2 and gcg expression. (A) InR1-G9 cells were treated with 100 μM H₂O₂ for 1 to 6 h, nuclear extract was prepared, followed by the analysis of Oct-1 and Cdx-2 expression by Western blotting. (B) The quantification results of Fig 5A. (C, D) InR1-G9 cells were treated with 100 μM H₂O₂ for 1 to 6 h. Cdx-2 (C) and gcg (D) mRNA expression was measured by real time RT-PCR.