Reduced level of A267T PC in transiently transfected cells. CHO-K1 (A) and Huh7 (B) cells were transiently transfected with plasmid constructs expressing WT or A267T PC. The culture medium ('white square') and cell lysates ('black square') were harvested 24 and 48 h after the transfection. The PC levels were measured using an ELISA kit and the PC levels in Huh7 were adjusted to the endogenous PC levels in these cells as described in Methods. The concentration of PC was adjusted to TP concentration in the corresponding sample. Histograms and the bars represent the mean and SEM values for CHO-K1 (n = 9) and for Huh7 cells (n = 8). * p < 0.05 (Mann-Whitney test, A267T PC versus WT PC).