Figure 8

Paracellular tracer flux assay. (A) changes in permeability to LY, Na-F or FITC-dextrans with molecular masses of 10 kDa, 20 kDa and 70 kDa were determined after stimulation with cAMP or its derivates for 24 to 72 h. (B) Endothelial monolayer were stimulated with the different cAMP derivates for 72 h. Paracellular permeability towards LY, FD20 and FD70 was determined after pretreatment of the cells with thrombin (2 U/mL). Shown are results from three independent experiments with HUVEC derived from different donors. (*p < 0.05 compared to untreated controls).