NOL7 contains three NLSs that are sufficient for nuclear localization. (A) Schematic representing the three different NLSs cloned in-frame with the cytoplasmic protein PK bearing a c-myc tag. Results demonstrated in (B) are summarized in the column to the right, where "Np" designates nucleoplasmic localization and "C" designates cytoplasmic localization. (B) Localization of the constructs in Hela cells was confirmed by immunofluorescence using an α-myc primary and Cy-3 conjugated secondary antibody. Costaining of the nucleus with DAPI is shown in blue.