Figure 3

Stress fiber formation is mediated by RhoA and Dbl. HeLa-S3 cells were transfected with siRNAs and then re-transfected 2 days later with myc-tagged wild type LKB1. A. HeLa-S3 cells transfected with control siRNA followed 2 days later by empty vector. B, C. Cells transfected with control siRNA followed 2 days later by a myc-tagged LKB1 construct. D, E. Cells transfected with RhoA siRNA SMARTpool followed 2 days later by a myc-tagged LKB1 construct. F, G. Cells transfected with Dbl SMARTpool siRNA followed 2 days later by a myc-tagged LKB1 construct. H. Quantification of stress fiber formation. Four individual siRNAs (1-4) and the SMARTpool (sp) are shown for RhoA and two individual siRNAs (1,2) and SMARTpool (sp) are shown for Dbl. I. Western blot showing effectiveness of individual siRNAs in depleting endogenous RhoA and Dbl proteins. Cell lysates were harvested 3 days after siRNA transfection. Scale bar = 20 μm.