Figure 5

Expression of LKB1 activates RhoA in HeLa-S3 cells. Cells were transfected with different LKB1 constructs and 24 hours after transfection, serum-starved for 12 hours. Cells were harvested and activated Rho. GTP levels were determined using a standard pull down assay. Wild type LKB1 and kinase dead LKB1 (D194A) induce a 3-4 fold increase in the levels of active RhoA relative to mock-transfected cells, whereas expression of a C-terminal fragment of LKB1 has no effect.