Figure 1From: PIST regulates the intracellular trafficking and plasma membrane expression of Cadherin 23Interaction between PIST and cadherin 23. A. Schematic diagram of chicken cadherin 23 domain structure and the intracellular fragment used as the bait for yeast two-hybrid screens. B. Schematic diagram of chicken PIST domain structure and PIST protein fragments encoded by the clones isolated from yeast two-hybrid screens. C. Western blots showing co-immunoprecipitation (co-IP) of EGFP-tagged human PIST protein with Myc-tagged mouse cadherin 23(+68) and cadherin 23(-68). The co-IP is almost undetectable when the last 4 amino acids (ITEL) of cadherin 23 are deleted. IP indicates antibody used for immunoprecipitation and WB indicates antibody used for detection.Back to article page