Figure 3

Analysis of cell cycle and cell senescence-associated β-galactosidase activity in BME65Cs and BMECs cells. A. Cell cycle analysis of BME65Cs cells (passage 102), BMECs cells primary passage 12 and 28 was performed using flow cytometry of propidium iodide-stained cells. A similar proportion of cells in S phase were observed in immortalised BME65Cs cells and early passage BMECs cells. B. Early passage 7, BMEC cells senescent passage 28 and immortal BME65Cs cells passage 87 were stained for β-galactosidase activity. Cells were fixed and incubated with X-gal. Blue staining indicates the presence of senescence associated β-galactosidase. This illustrates that a portion of immortalised BME65Cs cells (PDL 87) were in the senescent state. Bars: 20 μm.