Spontaneously immortalised bovine mammary epithelial cells exhibit a distinct gene expression pattern from the breast cancer cells

Table 1 Primer pairs used in the real-time PCR experiments of gene expression profiles Primer pairs applied for real-time PCR

Gene	Accession	Sense primer	Antisense primer	Product
bTERT	NM001046242	TCACGGGTCAAGACGCTGT	CGGGCATAACTGGAGTGGT	886 bp
p16^INK4a	XM868375	GGTGATGATGATGGGCAGCG	ACCAGCGTGTCCTGGAAGC	134 bp
p53	NM174201	CGGAACACCTTTAGACACAGT	GTAGGCAGTGCTCGCTTAGT	304 bp
p21^Cip1	NM001098958	TCTACCACTCCAAACGCA	GCAAAGGATGAAGAGGGTT	331 bp
TRPS-1	NM014112*	GCCTTCTTTGAGTTCGGA	CCCTCTGCTGTTTGTTGAG	384 bp
Bcl-2	XM586976	GCCTTCTTTGAGTTCGGA	TTCAGAGACAGCCAGGAGA	209 bp
Bax	NM173894	AGTGGCGGCTGAAATGTT	TTCTTCCAGATGGTGAGCG	287 bp
Bag-1	NM001076291	TGCCATTGTCAGCACTTG	TGTTCTGTTCCACTGTGTCAC	376 bp
c-Myc	AF519455	GTGTCTACCCATCAGCACAA	AACTGTTCTCGCCTCTTCTG	376 bp
DNMT1	NM182651	CCCGAGTGTGGAAAGTGTA	GAACATCTGCCCATTGCT	392 bp

*TRPS-1 primer pairs were designed by comparing homologous sequences between the Homo sapiens and Bos taurus genome, because the cattle TRPS-1 sequence has not been reported.
All other primer sequences were cited from GenBank. Each pairs of primers span intron-extron boundaries.

ISSN: 2661-8850