Figure 1From: Lipid droplets as ubiquitous fat storage organelles in C. elegansDifferent labelling properties of Nile Red and BODIPY in live animals. (A and B) Nile Red labelled intracellular structures in gut epithelial cells in wild-type (WT) animals but not in glo-4 mutants. Stained midlines were gut lumen (the same in other images if not otherwise indicated). (C and D) No autofluorescence in unstained wild-type or glo-4 animals was detected under the same Nile Red imaging condition. (E) BODIPY labelled a population of structures with high intensity (arrowheads) and labelled another population of structures with low intensity (white arrows) in gut epithelia cells in wild-type animals. (F) In glo-4 animals, only the low-intensity BODIPY structures remained in gut epithelial cells (arrows). Red arrows point to structures in hypodermal cells (E, F and J). (G and H) LRO-specific autofluorescence (arrows) was detected under the same BODIPY imaging condition in unstained wild-type animals but not in unstained glo-4 animals. (I and J) Nile Red and BODIPY labelled both enlarged lipid droplets (arrowheads) and putative small lipid droplets (white arrows) in live daf-22; glo-4 mutants. To better visualize Nile Red-stained structures, brightness of the original image (I, inset) was enhanced. All animals were late stage L4 if not other otherwise indicated. All images were 3-D projections of 9 μm confocal stacks. Gridlines, 10 μm.Back to article page