Carbon monoxide prevents hepatic mitochondrial membrane permeabilization

Table 1 Carbon monoxide prevents inner membrane permeabilization

	Control	Ca²⁺ 5 μM	Atra 300 μM	CO 10 μM	CO 10 μM Ca²⁺ 5 μM	CO 10 μM Atra 300 μM
Slope (Abs/min)	0,00E + 00	1,57E - 02	2,28E - 02	0,00E + 00	1,34E - 02	1,27E - 02
% ΔSlope (Abs/min) relative to Ca ²⁺	0,00	100,00 ± 1,02	145,13 ± 1,20	0,00	85,44 ± 0, 53	81,25 ± 0, 46

Inner membrane permeabilization is followed by absorbance at 412 nm. The slope was calculated between 10 to 20 minutes for each different condition in order to assess the effect of CO in the inner membrane permeabilization after treatment with Ca²⁺ or atractyloside. For slope calculation, absorbance values for each different condition were normalized by the absorbance value corresponding to the control non-treated mitochondria. The difference between control mitochondria and Ca²⁺ -treated mitochondria slopes correspond to an increase of 100%.

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