Carbon monoxide prevents hepatic mitochondrial membrane permeabilization

Table 2 Role of ROS in CO prevention of inner membrane permeabilization

	Control	Ca²⁺ 5 μM	CO 10 μM	CO 10 μM Ca²⁺ 5 μM	CO 10 μM Ca²⁺ 5 μM ß-c 1 μM
Slope (Abs/min)	0,00E + 00	1,78E - 02	00E00	9,60E - 03	1,34E - 02
% ΔSlope (Abs/min) relative to Ca ²⁺	0,00	100,00 ± 0,68	0,00	53,79 ± 0,56	75,22 ± 0,65

Inner membrane permeabilization is followed by absorbance at 412 nm. The slope was calculated between 10 to 20 minutes for each different condition in order to assess the effect of CO in the inner membrane permeabilization in the presence or absence of ß-carotene and after treatment with Ca²⁺. For slope calculation, absorbance values for each different condition were normalized by the absorbance value corresponding to the control non-treated mitochondria. The difference between control mitochondria and Ca²⁺ -treated mitochondria slopes correspond to an increase of 100%.

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