Determination of Elk-1 activities in SGC7901 cells. SGC7901 cells were transfected with either the pFR-Luc plasmid (reporter plasmid), or the pFA2-Elk1 plasmid (fusion trans-activator plasmid). Cells were then treated with ATX/LPC2, in the absence or presence of DMSO, Ki16425, PD98059, LY294002, harvested and the luciferase activity was measured. Statistical analysis was performed using Student's t test. *p < 0.05.