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Table 4 List of primers for amplification of lineage-specifics transcripts (GAPDH is used as control)

From: Long term culture of mesenchymal stem cells in hypoxia promotes a genetic program maintaining their undifferentiated and multipotent status

Gene Product Primers Product Size (bp)
GAPDH (NM_002046.3) Fw: 5'-AATCCCATCACCATCTTCCAGG-3'
Rv: 5'-AGAGGCAGGGATGATGTTCTGG-3'
417
ALPL
(NM_000478)
Fw: 5'-CTGGACCTCGTTGACACCTG-3'
Rv: 5'-GCGGTGAACGAGAGAATGTC-3'
546
LPL
(NM_000237.2)
Fw: 5'-AAAGCCCTGCTCGTGCTGAC-3'
Rv: 5'-ACAGGATGTGGCCCGGTTTA-3'
406
PPARG (NM_005037.5) Fw: 5'-GGAGAAGCTGTTGGCGGAGA-3'
Rv: 5'-CACAATGCTGGCCTCCTTGA-3'
431
RUNX-2 (NM_001015051.2) Fw: 5'-AACTTCCTGTGCTCGGTGCTG-3'
Rv: 5'-GGGGAGGATTTGTGAAGACGG-3'
268