Table 4 List of primers for amplification of lineage-specifics transcripts (GAPDH is used as control)
Gene Product | Primers | Product Size (bp) |
|---|---|---|
GAPDH (NM_002046.3) | Fw: 5'-AATCCCATCACCATCTTCCAGG-3' Rv: 5'-AGAGGCAGGGATGATGTTCTGG-3' | 417 |
ALPL (NM_000478) | Fw: 5'-CTGGACCTCGTTGACACCTG-3' Rv: 5'-GCGGTGAACGAGAGAATGTC-3' | 546 |
LPL (NM_000237.2) | Fw: 5'-AAAGCCCTGCTCGTGCTGAC-3' Rv: 5'-ACAGGATGTGGCCCGGTTTA-3' | 406 |
PPARG (NM_005037.5) | Fw: 5'-GGAGAAGCTGTTGGCGGAGA-3' Rv: 5'-CACAATGCTGGCCTCCTTGA-3' | 431 |
RUNX-2 (NM_001015051.2) | Fw: 5'-AACTTCCTGTGCTCGGTGCTG-3' Rv: 5'-GGGGAGGATTTGTGAAGACGG-3' | 268 |