Figure 5
From: Mitotic phosphorylation activates hepatoma-derived growth factor as a mitogen
Phosphorylation of S103 HDGF is increased during mitotic phase of cell division. A. MDA-MB231 cells were treated without (control) or with nocodazole (200 nM) to arrest the cell cycle at mitosis and immunostained with the anti-phospho-S103-HDGF antibody and DAPI. B. Graph representing percentage of phospho-S103-HDGF positive cells in control and nocodazole treated cells (n = 3; p = 0.037 calculated by unpaired two-tailed T test).