Figure 6
From: Mitotic phosphorylation activates hepatoma-derived growth factor as a mitogen
HDGF-S103A is not a mitogen. HDGF-GFP-S103A was expressed in mouse VSMC for 24 hours and cells labeled with BrdU overnight. Cells were immunostained for BrdU as a marker of DNA replication and observed for co-localization of GFP and BrdU. A = cells expressing HDGF-GFP-S103A, B = no staining for BrdU, C = DAPI staining to show that HDGF-GFP-S103A nuclear targeting is not affected.