Figure 3
TorA-ΔE localization in the NE requires SUN1. (A) SUN1 depletion selectively inhibits the NE-localization of (GFP)torA-ΔE. Images show anti-GFP labeling of NIH-3T3 cells co-transfected with (GFP)torA isoforms and control (left panels) or SUN1 siRNA duplexes (right panels). Scale bar shows 10 μm. (B - D) Only SUN1 siRNA reduces the ratio of NE/ER localized (GFP)torA-ΔE fluorescence. Columns show the mean and s.e.m. of the relative NE/ER GFP fluorescence from n > 25 NIH-3T3 cells transfected with (GFP)torA isoforms alongside (B) SUN1, (C) SUN2, or (D) Nesprin2 siRNA duplexes, or a sequence matched scrambled control siRNA duplex. The ** and *** symbols indicate a significant (p < 0.01, < 0.001, respectively) difference between control and siRNA treated cells. (Two-Way ANOVA with Bonferroni post-hoc analysis.)