Figure 5
From: BMP signaling balances proliferation and differentiation of muscle satellite cell descendants
Inhibition of BMP signaling triggers immediate cell cycle exit of satellite cells. (A) Prior to the induction of differentiation, cultures of proliferating satellite cells were supplemented for 2 hours with BrdU and cultured for 3 days in differentiation medium. Myonuclei within MHC-positive myotubes from control cultures (B-C) show weak BrdU signals, while Noggin-treated myonuclei (D-E) show BrdU signals of stronger intensity in MHC-positive myotubes. (F) Analysis of the mean fluorescence intensity of the BrdU signal of Noggin-treated cultures (red columns; n = 92 myonuclei) compared to control cultures (black columns; n = 117 myonuclei). Histogram showing a shift in the frequency of Noggin-treated cells to higher levels of BrdU (p < 0,005; Wilcoxon test).