Figure 1

Redistribution of flotillin-1 and -2, PSGL-1 and P-ERM upon stimulation of human T-lymphoblasts in suspension. (A-C) After an overnight incubation in the absence of IL-2, cells were incubated either for 45 min at 37°C (control) or were stimulated with 40 ng/ml SDF-1 for 1 or 15 minutes after a 44 or 30 minutes preincubation at 37°C (total incubation time 45 minutes). Cells were then fixed with 10% TCA and double-labeled for flotillin-2 (flo2; rabbit Ab) and PSGL-1 (murine Ab) in (A) or for flotillin-1 (flo1; murine Ab) and P-ERM (rabbit Ab) in (B). or for flotillin-1 (murine Ab) and flotillin-2 (rabbit Ab) in (C). Asterisks indicate cells shown enlarged in the panels labelled "detail". Dic; differential interference contrast. Bar, 10 μm. Note that in panels C overlays only are shown.