Dynamic reorganization of flotillins in chemokine-stimulated human T-lymphocytes

Table 1 FRAP analysis of the lateral mobility of flotillins in T-lymphocytes

Transfection	Cell type	M_F - SDF-1 (n)	M_F + SDF-1 (n)
flo1-EGFP	T-lymphoblast	n.d.	0.88 ± 0.05 (12)
flo1-EGFP/flo2-mCherry	T-lymphoblast	n.d.	0.04 ± 0.01 (10)
flo2-EGFP	T-lymphoblast	n.d.	0.80 ± 0.06 (7)
flo2-EGFP/flo1-mCherry	T-lymphoblast	n.d.	0.06 ± 0.02 (7)
flo2-EGFP	Freshly isolated T-cells	0.79 ± 0.04 (14)	0.71 ± 0.03 (15)
flo2-EGFP/flo1-mCherry	Freshly isolated T-cells	0.38 ± 0.04 (16)	0.11 ± 0.02 (11)

FRAP analysis involving local bleaching of EGFP was carried out as described in the legend of Figure 6 with cells preincubated in the absence or presence of SDF-1, as indicated. The mobile fraction (M_F) was calculated for each individual cell and then averaged from a total of n = 7-16 cells, as indicated (mean ± s.e.m.) (data obtained in 2-4 independent experiments). N.d. = not done.

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