Dynamic reorganization of flotillins in chemokine-stimulated human T-lymphocytes

Table 4 Impact of pretreatment with blebbistatin on the lateral mobility of flotillin-2 cotransfected with flotillin-1 in freshly isolated T-lymphocytes

Transfection	Pretreatment	M_F - SDF-1 (n)	M_F + SDF-1 (n)
flo2-mCherry/flo-1-EGFP	No pretreatment	0.43 ± 0.05 (11)	0.11 ± 0.03 (13)
flo2-mCherry/flo1-EGFP	Preincubation for 30 min with blebbistatin (100 μM)	0.49 ± 0.06 (9)	0.09 ± 0.02 (10)

FRAP analysis involving local bleaching of mCherry was carried out as described in the legend of Figure 6 with cells preincubated in the absence or presence of SDF-1, and without or with pretreatment with blebbistatin as indicated. The mobile fraction (M_F) was calculated for each individual cell and then averaged from a total of n = 9 - 13 cells observed in 3 independent experiments, as indicated (mean ± s.e.m.).

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