Figure 4
Extracellular matrix derived from mouse embryonic fibroblasts (MEF-ECM) improves proliferation of mMSCs at early passage. (A) Morphology of mMSCs at passage one cultured on plastic and MEF-ECM, respectively. (B) Population doublings of mMSCs cultured with or without MEF-ECM. mMSCs were plated at 1000 cells/cm2 on 6-well dishes coated with or without MEF-ECM and incubated for 4 days, and cells were counted by hemocytometer. (C) ROS production by mMSCs cultured on plastic or MEF-ECM, measured by CM-H2DCFDA fluorescence. (D) Comparison of DNA double-strand breaks in mMSCs cultured on plastic or MEF-ECM, determined by flow cytometry analysis of γ-H2AX fluorescence intensity. (E) Spontaneous micronucleus formation in mMSCs cultured on plastic or MEF-ECM. Data are expressed as the mean ± SD. *p < 0.05; **p < 0.01; Scale bar = 100 μm.