Isolation of mouse mesenchymal stem cells with normal ploidy from bone marrows by reducing oxidative stress in combination with extracellular matrix

Table 1 Karyotypes of mMSCs cultured under 2% O₂

Number of chromosomes	< 40	40	41-79	80	> 80	Total number of metaphases	Normal karyotype (%)
Passage 1
CmMSCs P1	3	15	13	2	17	50	30
EmMSCs P1	6	28	10	2	4	50	56
Primary cultures
CmMSCs P0	2	12	15	8	13	50	24
PCmMSCs P0	5	31	6	2	6	50	62
PEmMSCs P0 (low density)	6	42	0	2	0	50	84
Passage 8
CmMSCs P8	0	0	45	0	5	50	0
EmMSCs P8	1	0	43	2	4	50	0
PCmMSCs P8	1	1	2	0	46	50	2
PEmMSCs P8	3	14	29	1	3	50	28

Abbreviations: CmMSCs, mMSCs on control plastic; EmMSCs, mMSCs on MEF-ECM; PCmMSCs, mMSCs on control plastic in the presence of PBN and NAC; PEmMSCs, mMSCs on MEF-ECM in the presence of PBN and NAC.

ISSN: 2661-8850