Myosin II activity and vinculin recruitment to cell-cell contacts of MDCK cells. (A) Confluent MDCK cell monolayers on collagen-coated coverslips were incubated with media containing 50 μM blebbistatin (Bleb), 10 nM calyculin-A (CA), or media alone (Control) for 1 hr. Cells were fixed and immunostained for vinculin or F-actin (phalloidin) at cell-cell contacts. The same exposure and laser power were used to acquire and generate the images. Scale bar 10 μm. Data of immuno-fluorescence intensities and analysis of F-actin and vinculin at cell-cell contacts are presented as mean ± standard deviation and are compared with control (n = 10, *P < 0.05, ***P < 0.001). (B) Immuno-fluorescence of vinculin and F-actin at basal level of confluent MDCK cells. Scale bar 10 μm.