Figure 2

Expression, localization and assembly of MHC IIA during cell division. (A) Whole cell lysates of HeLa cells expressing the indicated RLC construct were probed for total RLC, GFP and MHC IIA. (B) MHC IIA was immunoprecipitated from the indicated cell line and the eluate was probed for MHC IIA, RLC and GFP. Note that the RLC-GFP and IgG heavy chain migrate too close to differentiate. The number under each construct indicates the relative amount of RLC present after normalization to MHC IIA based on densitometry. (C) Anaphase cells expressing the indicated RLC construct (green) were fixed and stained for MHC IIA (red). Representative images are shown. (C) MHC IIA recruitment was quantitated for a minimum of 13 cells. Data are mean +/- SEM. * indicates p = < 0.003.