Figure 5
Mouse ESCneo differentiation in suspension culture. A) Murine ES cell aggregates. The size of EBs was growing over time period of differentiation. B) Beating cardiac body (CB) 21 days after differentiation. The CBs were beating rhythmically with various rates. C) Percentage of beating EBs. The maximum percentage of beating was 50% on day 14 of EBs. D) Dissociated cardiomyocytes stained with specific cardiac antibody Myosin sarcomere (MF-20) green and DAPI-blue. E) Beating cardiac bodies showed specific subcellular ultrstructure of cardiomyocytes using electron microscopy. The presence the cardiomyocytes specific structure including nucleus and myofibrils, mitochondria, Endoplasmic reticulum, gap junctions and desmosomes was shown. Hb: H-band; Ib: I-Band; Ab: A-band; Zb: Z-band; Dj: Desmosome Junction; GJ: Gap junction; Mi: Mitochondria; RE: Reticulum Endoplasmic; N: Nucleus. Scale bars: 100 μM.