KlarMW is expressed in embryos, but not localized to lipid droplets. (A) Klar Western of embryos (cycle 14/Phase II) of various genotypes. Since klarMW and klarZ are present on TM6B balancer chromosomes, these alleles were analyzed in combination with Df(3L)emcE12, a deletion that encompasses the entire klar locus. The KlarMW protein is expressed and stable, as it accumulates to similar levels as the wild-type protein KlarZ. (B) Embryos were centrifuged to separate organelles by density, fixed and stained for Klar. The lipid-droplet layer was recognized by its distinctive appearance by bright-field microscopy. In this image, embryos are arranged with the lipid-droplet layer pointing up. Klar signal is enriched on the droplet layer in klarZ embryos, but not detectable on the droplet layer when the LD domain is truncated (klarMW) or Klar β is not expressed (klarYG3).