Figure 2

Klar^MW is expressed in embryos, but not localized to lipid droplets. (A) Klar Western of embryos (cycle 14/Phase II) of various genotypes. Since klar^MW and klar^Z are present on TM6B balancer chromosomes, these alleles were analyzed in combination with Df(3L)emc^E12, a deletion that encompasses the entire klar locus. The Klar^MW protein is expressed and stable, as it accumulates to similar levels as the wild-type protein Klar^Z. (B) Embryos were centrifuged to separate organelles by density, fixed and stained for Klar. The lipid-droplet layer was recognized by its distinctive appearance by bright-field microscopy. In this image, embryos are arranged with the lipid-droplet layer pointing up. Klar signal is enriched on the droplet layer in klar^Z embryos, but not detectable on the droplet layer when the LD domain is truncated (klar^MW) or Klar β is not expressed (klar^YG3).