Figure 2

RNAi knockdown of UBCs affects the cellular level and aggregation rate of Q82::GFP fusion protein. C. elegans expressing a Q82:GFP transgene were fed bacterial clones expressing dsRNA against the indicated genes or the empty pL4440 vector as a control, beginning at the L2 stage. The progeny of these worms were imaged at a rate of 1 frame per minute. Pixel intensity in a square region of the image sequence in which an aggregate formed was measured over time. Plots of individual formation events were aligned along the time axis so that the frame at which the aggregation rate is highest occurs at 60 minutes. This chart shows the mean (± SEM) aggregate intensity at each time point for each RNAi treatment. At least 50 aggregates were analyzed for each RNAi treatment. See Table 1 for calculation of aggregation rates.