Table 1 Strains used in this study

YJR12,YJR24_1 (wild type, wt)

MAT α trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyh^R

Lab. Strain

JK9-3da (wt’)

MAT a leu2-3,112 trp ura3 rmel his4 HMLa

M. Hall

YJR13 (myo1 Δ)

MAT a trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyh^Rmyo1Δ::HIS5+

Lab. Strain

YFR22 (fks1 Δ)

MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyh^Rfks1 Δ::kanMX4

F. Rivera

YFR23 (chs2 Δ)

MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyh^Rchs2 Δ::kanMX4

F. Rivera

YJR066W (tor1 Δ)

MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 tor1Δ::kanMX4

B. Rymond

YGP1 (myo1 Δtor1 Δ)

MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 tor1Δ::kanMX4, myo1Δ::HIS5+

This study

YOR008C (wsc1 Δ)

MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 wsc1Δ::kanMX4

B. Rymond

YES1 (myo1 Δwsc1 Δ)

MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 wsc1Δ::kanMX4, myo1Δ::HIS5+

E. Santiago

MH346-1a/pJK3-3

JK9-3da ade2 tor2::ADE2/pSEY18::TOR2

M. Hall

SH121 (tor2 Δ ptor2^ts)

JK9-3da ade2 tor2::ADE2/YCplac111::tor2-21^ts

M. Hall

YGP5 (myo1 Δtor2 Δptor2^ts)

JK9-3da ade2 tor2::ADE2/YCplac111::tor2-21^tsmyo1 Δ::HIS5+

This study

YJR12/pEJ23 (wt pHA-NPR1)

MAT α trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyh^R, pHA-NPR1

This study

YJR13pEJ23 (myo1Δ pHA-NPR1)

MAT a trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyh^R, myo1 Δ::HIS5 + pHA-NPR1

This study

(YFR22pEJ23) (fks1 Δ pHA-NPR1)

MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyh^Rfks1 Δ::kanMX4, pHA-NPR1

This study

YFR23pEJ23 (chs2 ΔpHA-NPR1)

MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyh^Rchs2 Δ::kanMX4, pHA-NPR1

This study

YJR066WpEJ23 (tor1 ΔpHA-NPR1)

MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 tor1Δ::kanMX4, pHA-NPR1

This study

YGP1pEJ23 (myo1 Δtor1 Δ pHA-NPR1)

MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 tor1Δ::kanMX4 myo1 Δ::HIS5+, pHA-NPR1

This study

YDL047W pEJ23 (sit4 ΔpHA-NPR1)

MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 sit4Δ::kanMX4, pHA-NPR1

This study

YGP3pEJ23 (myo1Δsit4 Δ pHA-NPR1)

MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 sit4Δ::kanMX4 myo1 Δ::HIS5+, pHA-NPR1

This study

YOR008C pEJ23 (wsc1 ΔpHA-NPR1)

MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 wsc1Δ::kanMX4, pHA-NPR1

This study

YES1 pEJ23 (myo1 Δwsc1 Δ pHA-NPR1)

MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 wsc1Δ::kanMX4 myo1 Δ::HIS5+, pHA-NPR1

This study

YJR24_1/YCplac111 (wt ptor2^ts)

MAT α trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyh^R, YCplac111::tor2-21^ts

This study

YJR13/ YCplac111 (myo1 Δ ptor2^ts)

MAT a trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyh^Rmyo1 Δ::HIS5+, YCplac111::tor2-21^ts

This study

YFR23/p YCplac111 (chs2 Δptor2^ts)

MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyh^Rchs2 Δ::kanMX4, YCplac111::tor2-21^ts

This study

YJR13YCplac111pRS316MYO1 (myo1 Δptor2^tspMYO1)

MAT a trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyh^Rmyo1 Δ::HIS5+, YCplac111::tor2-21^ts pRS316MYO1

This study

YGP5pRS316MYO1 (myo1 Δtor2 Δptor2^tspMYO1)

JK9-3da ade2 tor2::ADE2, myo1 Δ::HIS5+, YCplac111::tor2-21^ts pRS316MYO1

This study