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Table 1 Strains used in this study

From: Functional and genetic interactions of TOR in the budding yeast Saccharomyces cerevisiae with myosin type II-deficiency (myo1Δ)

Strain Genotype Source
YJR12,YJR24_1 (wild type, wt) MAT α trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhR Lab. Strain
JK9-3da (wt’) MAT a leu2-3,112 trp ura3 rmel his4 HMLa M. Hall
YJR13 (myo1 Δ) MAT a trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhRmyo1Δ::HIS5+ Lab. Strain
YFR22 (fks1 Δ) MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyhRfks1 Δ::kanMX4 F. Rivera
YFR23 (chs2 Δ) MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyhRchs2 Δ::kanMX4 F. Rivera
YJR066W (tor1 Δ) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 tor1Δ::kanMX4 B. Rymond
YGP1 (myo1 Δtor1 Δ) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 tor1Δ::kanMX4, myo1Δ::HIS5+ This study
YOR008C (wsc1 Δ) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 wsc1Δ::kanMX4 B. Rymond
YES1 (myo1 Δwsc1 Δ) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 wsc1Δ::kanMX4, myo1Δ::HIS5+ E. Santiago
MH346-1a/pJK3-3 JK9-3da ade2 tor2::ADE2/pSEY18::TOR2 M. Hall
SH121 (tor2 Δ ptor2ts) JK9-3da ade2 tor2::ADE2/YCplac111::tor2-21ts M. Hall
YGP5 (myo1 Δtor2 Δptor2ts) JK9-3da ade2 tor2::ADE2/YCplac111::tor2-21tsmyo1 Δ::HIS5+ This study
YJR12/pEJ23 (wt pHA-NPR1) MAT α trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhR, pHA-NPR1 This study
YJR13pEJ23 (myo1Δ pHA-NPR1) MAT a trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhR, myo1 Δ::HIS5 + pHA-NPR1 This study
(YFR22pEJ23) (fks1 Δ pHA-NPR1) MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyhRfks1 Δ::kanMX4, pHA-NPR1 This study
YFR23pEJ23 (chs2 ΔpHA-NPR1) MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyhRchs2 Δ::kanMX4, pHA-NPR1 This study
YJR066WpEJ23 (tor1 ΔpHA-NPR1) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 tor1Δ::kanMX4, pHA-NPR1 This study
YGP1pEJ23 (myo1 Δtor1 Δ pHA-NPR1) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 tor1Δ::kanMX4 myo1 Δ::HIS5+, pHA-NPR1 This study
YDL047W pEJ23 (sit4 ΔpHA-NPR1) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 sit4Δ::kanMX4, pHA-NPR1 This study
YGP3pEJ23 (myo1Δsit4 Δ pHA-NPR1) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 sit4Δ::kanMX4 myo1 Δ::HIS5+, pHA-NPR1 This study
YOR008C pEJ23 (wsc1 ΔpHA-NPR1) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 wsc1Δ::kanMX4, pHA-NPR1 This study
YES1 pEJ23 (myo1 Δwsc1 Δ pHA-NPR1) MAT α his3Δ1 leu2Δ0 lysΔ0 ura3Δ0 wsc1Δ::kanMX4 myo1 Δ::HIS5+, pHA-NPR1 This study
YJR24_1/YCplac111 (wt ptor2ts) MAT α trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhR, YCplac111::tor2-21ts This study
YJR13/ YCplac111 (myo1 Δ ptor2ts) MAT a trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhRmyo1 Δ::HIS5+, YCplac111::tor2-21ts This study
YFR23/p YCplac111 (chs2 Δptor2ts) MAT α trp1-289 ura3-52 leu2-3, 112 his3Δ1 ADE + ARG + cyhRchs2 Δ::kanMX4, YCplac111::tor2-21ts This study
YJR13YCplac111pRS316MYO1 (myo1 Δptor2tspMYO1) MAT a trp1 ura3 leu2-3 his3Δ1 met- ADE + ARG + cyhRmyo1 Δ::HIS5+, YCplac111::tor2-21ts pRS316MYO1 This study
YGP5pRS316MYO1 (myo1 Δtor2 Δptor2tspMYO1) JK9-3da ade2 tor2::ADE2, myo1 Δ::HIS5+, YCplac111::tor2-21ts pRS316MYO1 This study