Figure 2From: Characterization of the C. elegans erlin homologueERL-1 association into high MW complexes depends on Phe-303. Sucrose density gradient centrifugation was performed on HEK293 cells transiently transfected with wild type (upper panel) or F303A (lower panel) ERL-1HA. Twelve fractions were collected from each gradient, which were analyzed by Western blotting using an HA-tag specific antibody.Back to article page