Effects of wild-type and chimeric proteins on in vitro cell growth (A-D) and gene expression (E-G). Cells were plated and cultured for 2 days in the growth medium containing 30 μg/ml proteins. (A)Wild-type TC14-3. (B) TC14-221-60/TC14-361-145. (C)Wild-type TC14-2. (D)TC14-321-60/TC14-261-145. Bar, 50 μm. (E-G)Agarose gel staining of RT-PCR products (arrowheads). Lanes 1-5, TC14-3E106G. Lanes 6-10, wild-type TC14-3. (E)Pmβ-actin. (F)PmCyclin A. (G)PmCyclin B.