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Figure 6 | BMC Cell Biology

Figure 6

From: Tunicate cytostatic factor TC14-3 induces a polycomb group gene and histone modification through Ca2+ binding and protein dimerization

Figure 6

Quantification of in vitro and in vivo PmEed induction by wild-type and mutant TC14-3s. (A, B)Cultured cells. (A)PCR products of PmEed (upper) and Pmβ-actin (lower). Lane 1, control (PBS). Lane 2, wild-type TC14-3. Lane 3, TC14-3T69R. Lane 4, TC14-3E106G. (B)PCR products from 25th to 30th cycle. Lanes 1-6, control (PBS). Lanes 7-12, wild-type TC14-3. (C-E)Aged zooid pieces. (C)PCR products of PmEed (upper) and β-actin (lower). Lane 1, control (PBS). Lane 2, wild type TC14-3. Lane 3, TC14-3K113S.N114E. Lane 4, TC14-3T69R. Lane 5, TC14-3E106G. (D)PCR products from 25th to 30th cycle. Lanes 1-6, control (PBS). Lanes 7-12, wild-type TC14-3. (C)Increasing kinetics of PCR products quantified by ImageJ output.

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