Surface expression of TRPC6. HEK293T cells were transiently transfected with the indicated combinations of FLAG-TRPC6 (FLAG-C6) and HA-SNF8 expression plasmids. 48 hours after transfection, cells were surface biotinylated or mock biotinylated (-) as indicated and lysed. Biotinylated protein and associated proteins were isolated by binding to streptavidin agarose beads. The amounts of TRPC6 and SNF8 in whole lysate (left panel) and bound to streptavidin beads (right panel) were assessed by Western blotting.