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Table 1 Characteristics of the real-time PCR assays used in the study

From: Pressure mediated hypertrophy and mechanical stretch up-regulate expression of the long form of leptin receptor (ob-Rb) in rat cardiac myocytes

Target Species Sequence ACC No.
ob Rat forward 5-CCAAAACCCTCATCAAGACC-3 MN013076
   reverse 5-GTCCAACTGTTGAAGAATGTCCC-3  
ob-Ra Rat forward 5-ACACTGTTAATTTCACACCAGAG-3 D85557
   reverse 5-AGTCATTCAAACCATAGTTTAGG-3  
ob-Rb Rat forward 5-TCTTCTGGAGCCTGAACCCATTTC-3 D85558
   reverse 5-TTCTCACCAGAGGTCCCTAAACT-3  
GAPDH Rat forward 5-AACGACCCCTTCATTGAC-3 MN017008
   reverse 5-TCCACGACATACTCAGCAC-3  
  1. Ob: leptin, ob-Ra –Rb; leptin receptor isoforms.