| | HEKOPRM1 | HEKC170A |
---|
Relative Affinities
| | |
Morphine |
K
H
(nM)
| 2.8 ± 0.56 | 2.2 ± 0.42 |
|
K
L
(nM)
| 155 ± 27 | 123 ± 31 |
Naloxone |
K
I
(nM)
| 5.1 ± 0.71 | 6.2 ± 1.2 |
CTOP |
K
I
(nM)
| 11 ± 1.4 | 9.5 ± 2.1 |
- The affinities of receptor for ligands were determined in HEKOPRM1 and HEKC170A cells using an agonist binding assay. One-site (KI: naloxone and CTOP) or two-site (KH and KL: morphine) curve-fitting models were used in the analyses with GraphPad Prism 5.0. Data were analyzed by one-way ANOVA with post-hoc Dunnett's test. Standard deviations are provided, and "*" represents significant changes (p < 0.05).