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Table 1 Palmitoylation does not affect the binding of agonists.

From: Palmitoylation and membrane cholesterol stabilize μ-opioid receptor homodimerization and G protein coupling

  

HEKOPRM1

HEKC170A

Relative Affinities

  

   Morphine

K H (nM)

2.8 ± 0.56

2.2 ± 0.42

 

K L (nM)

155 ± 27

123 ± 31

   Naloxone

K I (nM)

5.1 ± 0.71

6.2 ± 1.2

   CTOP

K I (nM)

11 ± 1.4

9.5 ± 2.1

  1. The affinities of receptor for ligands were determined in HEKOPRM1 and HEKC170A cells using an agonist binding assay. One-site (KI: naloxone and CTOP) or two-site (KH and KL: morphine) curve-fitting models were used in the analyses with GraphPad Prism 5.0. Data were analyzed by one-way ANOVA with post-hoc Dunnett's test. Standard deviations are provided, and "*" represents significant changes (p < 0.05).