Figure 3
Soft agar assay, tumour formation and immunohistochemistry. (A) Foci of colonies of GFP-aurA, GFP-aurC-CA, GFP-aurC-WT stable cell lines and very negligible number of very small colonies of GFP-alone in soft agar assay. (B) Histogram of the average number of colonies. (C) Visualization of the tumours formed by injecting GFP-aurC-CA and GFP-aurC-WT stable cell lines, and the remaining injected cells of GFP-alone on the day of sacrifice using Kodak image station 2000. (D) Rabbit monoclonal KI-67, a proliferation marker from late G1 to M-phase staining (E) anti-phospho histone-H3 ser-10 (Millipore) and anti-HRP (Jackson) secondary antibodies and (F & G) Feulgen staining showing prometaphase defects, metaphase defects, lagging chromosomes at anaphase, and cytokinesis defect.